As a result of collaboration with Prof. Elżbieta Żądzińska team, several studies have been conducted and among them these which were carried out with participation of the BioBank Lab e.g. storage of the collected biological material, DNA isolation, designing of methods for polymorphisms determination in FTO gene (genotyping by HRM method and direct sequencing), execution of genetic tests. Research results have been published in Anthropological Review (7 points of the Ministry of Science and Higher Education ) in OPEN ACCESS status. Enjoy your reading.

"Association of FTO gene withobesity in Polish schoolchildren"

Aneta Sitek1, Iwona Rosset1, Dominik Strapagiel2, Małgorzata Majewska2, Lidia Ostrowska-Nawarycz3, Elżbieta Żądzińska1

1Department of Anthropology, Faculty of Biology and Environmental Protection, University of Łódź, Poland

2Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Poland

3Department of Biophysics, Chair of Basic and Pre-clinical Sciences, Medical University in Łódź, Poland

 

 

ABSTRACT: The goal of the study was verification of fat mass and obesity-associated (FTO) gene polymorphisms as significant risk factors of obesity in the population of Polish children. Body mass index (BMI) and DNA were evaluated, where DNA was extracted from saliva, collected from 213 children at the age of 6-13 years. DNA was genotyped by PCR (polymerase chain reaction) and HRM (high resolution melting) techniques, as well as by direct sequencing. Three (3) FTO polymorphisms were identified: rs9939609, rs9926289 and rs76804286, the last polymorphism located between the first two. For the first time, absolute linkage disequilibrium (LD) of FTO gene rs9939609 and rs9926289 polymorphisms was confirmed in data for the Polish population (D’=1, r2=1). The lack of a complete dependence among the three single nucleotide polymorphisms (SNPs) of the FTO gene was a consequence of the concurrence of homozygotes with minor alleles A of rs9939609+rs9926289 of FTO (AA+AA) with major alleles of rs76804286 (GG). A case-control association analysis for BMI in obese children (n=51), as compared to normal-weight children (n=162), was based on the effects of genotypes homozygous for the minor alleles of the studied SNPs in recessive and codominant inheritance models (assuming an independent effect of each genotype). A comparison of children with normal BMI with obese children indicate a strong co-dominant effect of a genotype in homozygotes of minor alleles (AA+AA) of completely linked rs9939609+rs9926289 (OR at age 8.89 ± 1.54 years=4.87, 95% CI 1.81-13.12, p=0.002). An almost five-fold increase of obesity risk in the examined children indicates that the genetic factors, associated with excessive body weight gain, exert stronger effects in the early period of ontogenetic development vs. puberty and adulthood. The role of genetic factors in predisposing to obesity declines with age.

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